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Protease assays

Protease specificity and protease activity can be assayed by measuring the cleavage rate of protein substrates. One method is to incubate the protease with a protein substrate that has been modified with a large number of fluorophores. Since many fluorophores on the same molecule result in fluorescent quenching, the cleavage of these molecules causes an increase in fluorescence.

Let Protein Mods generate fluorescent substrates for your protease-assay needs. As we typically place a small number of fluorophores on our proteins, please let us know if you want a protein with much higher levels of modification.

At the moment, we offer one common protease substrate as a catalog item:

Highly modified Fluorescein BSA.