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Quantify GSH/GSSG Redox in Live Cells with SEMKUR IM

Overview: What is SemKur IM?

SemKur IM is a non-toxic, cell-permeable fluorescent probe for real-time imaging of redox states in live cells. It’s optimized to measure glutathione dynamics—specifically the GSH/GSSG ratio—under physiological conditions. Researchers use SemKur IM to visualize oxidative stress and track redox homeostasis without damaging the cell or disrupting its native environment.


Key Benefits

Real-Time Redox Imaging
SemKur IM detects rapid shifts in redox balance in response to drugs, stressors, or environmental stimuli. You can monitor transient signaling events that other probes may miss.

Reversible Disulfide Exchange
Unlike many redox indicators, SemKur IM uses reversible chemistry. This means it accurately reflects redox buffering capacity—not just static GSH levels.

Non-Toxic & Photostable
SemKur IM does not stress cells. Its design enables extended live-cell imaging without introducing artifacts, making it perfect for time-lapse studies.


Applications

SemKur IM is ideal for:

  • Live-cell redox imaging
  • Cell viability assays
  • Disease modeling (e.g., Alzheimer’s, cardiovascular, inflammatory disorders)
  • Drug screening and toxicity studies
  • Oxidative stress analysis

Researchers have validated SemKur IM in a range of cell types, including:

  • Neuronal cells
  • Immune cells
  • Epithelial cells
  • iPSC-derived cultures
  • Bacterial systems

How to Use SemKur IM

Protocol Overview
SemKur IM is typically used at 0.5 to 20 µM in standard in vitro assays. After adding to live cells, incubate for 10 to 60 minutes, depending on the system.

Detection

  • Microscopy: Capture fluorescence in real time with live-cell imaging platforms.
  • Plate Reader: Measure using a microplate reader (Ex: 485–495 nm / Em: 516–525 nm).

Time-course measurements (e.g., 0, 5, 15, 30, 60 minutes) allow you to map the full kinetics of redox shifts.


Compatibility

SemKur IM works across a wide range of media, buffers, and workflows. It is compatible with standard imaging platforms and instruments used in:

  • High-content screening
  • Confocal microscopy
  • Live-cell imaging systems
  • Automated plate readers
  • Flow Cytometry

Explore Redox with Confidence

SemKur IM’s unique DSSQ-based chemistry offers unmatched reliability for redox biology. Whether you’re investigating oxidative damage or screening for redox-active compounds, SemKur IM provides the resolution and reproducibility you need.