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Avidin and Streptavidin Reagents

Avidin is a tetrameric protein commonly isolated from egg whites. Avidin binds to biotin with strength approaching that of a covalent bond. Disruption of the avidin-biotin bond occurs only under extraordinary conditions, such as pH 1.5 with 6 M guanidium hydrocholoride. Fluorescent avidin can bind to biotinylated antibodies used in immunohistochemistry and immunocytochemistry. Avidin thiol can bind to gold surfaces and act as a glue for biotinylated proteins. Avidin agarose is useful for efficient removal of biotinylated proteins from a liquid phase.

Streptavidin is similar to avidin in that it binds biotin and exists as a tetramer. Streptavidin’s primary advantage is that it less charged under neutral conditions and is therefore less likely to be involved in unwanted ionic interactions.

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    Streptavidin azide

    Price range: $199.10 through $579.00
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    Neutravidin thiol

    Price range: $247.50 through $464.20
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    Streptavidin (without modification)

    Price range: $52.80 through $161.70
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    Streptavidin CY5.5

    Price range: $82.50 through $161.70
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    Streptavidin CY5

    Price range: $82.50 through $161.70
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    Streptavidin CY3

    Price range: $82.50 through $161.70
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    Streptavidin TRITC

    Price range: $82.50 through $156.20
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    Streptavidin-fluorescein (FITC)

    Price range: $49.00 through $105.00
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    Streptavidin thiol

    Price range: $199.10 through $776.60
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    Avidin CY3

    Price range: $88.00 through $187.00
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    Avidin CY5

    Price range: $88.00 through $187.00
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    Avidin (without modification)

    Price range: $25.30 through $327.80
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    Avidin Sepharose

    Price range: $66.00 through $379.50
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    Avidin thiol

    Price range: $88.00 through $819.50
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    Avidin CY5.5

    Price range: $88.00 through $434.50
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    Avidin-fluorescein

    Price range: $55.00 through $550.00
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