Easily evaluate cell vitality in real-time using Semkur-IM. Semkur-IM permeates live cells and fluoresces when the quenching component is cleaved off by cellular glutathione. Fluorescence intensity correlates with reduced glutathione (GSH) and cell vitality. The reduced/oxidized ratio of glutathione typically exceeds 10:1 in healthy cells. Abnormal levels are associated with numerous pathologies including cancer, neurodegenerative diseases, cystic fibrosis, cardiovascular diseases, HIV and aging.
- Cell permeable
- Has negligible effect on GSH levels
- Use with common fluorescent optics (identical excitation and emission maxima as fluorescein)